继发性萎缩性鼻炎的鼻黏膜蛋白质组学分析

Proteomic analysis of nasal mucosa in secondary atrophic rhinitis

摘要:

目的筛选继发性萎缩性鼻炎患者鼻黏膜组织的特异性差异表达蛋白(DEPs)，探讨继发性萎缩性鼻炎发生、发展的蛋白质水平分子机制。方法定量分析继发性萎缩性鼻炎患者鼻黏膜组织与上颌窦囊肿患者正常鼻黏膜组织的蛋白质表达谱，以差异蛋白表达倍数(FC)＞1.2或＜0.83且P＜0.05筛选DEPs，并对DEPs进一步进行GO和KEGG富集分析。结果共筛选出291个DEPs，其中53个上调，238个下调；GO富集分析显示DEPs主要参与内质网-高尔基体囊泡运输、核糖体的结构组成部分以及细胞质核糖体大亚基；KEGG富集分析显示DEPs主要参与鞘糖脂生物合成以及蛋白质的运输、加工等通路。结论通过4D-Label-free技术筛选出了继发性萎缩性鼻炎患者鼻黏膜组织的特异性DEPs，其中差异表达明显的蛋白质COL6A6、MPP1、GOLGA4以及SLC38A10可能在继发性萎缩性鼻炎的发生和发展中起重要作用。

Abstract:

Objective To identify specific differentially expressed proteins (DEPs) in the nasal mucosa of patients with secondary atrophic rhinitis and explore the protein-level molecular mechanism of the occurrence and progression of this disease. Methods The protein expression profiles of nasal mucosa tissues from patients with secondary atrophic rhinitis and normal nasal mucosa tissues from patients with maxillary sinus cysts were quantitatively analyzed. DEPs were identified based on fold change (FC) >1.2 or<0.83 and P<0.05, followed by gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses. Results A total of 291 DEPs were identified, including 53 upregulated proteins and 238 downregulated ones. GO enrichment analysis revealed that DEPs were mainly involved in processes such as endoplasmic reticulum-Golgi vesicle transport, structural components of ribosomes, and cytoplasmic large ribosomal subunits. KEGG enrichment analysis showed that DEPs were primarily involved in pathways related to sphingolipid biosynthesis, protein transport and processing. Conclusions Using 4D-label-free technology, specific DEPs are identified in the nasal mucosa of patients with secondary atrophic rhinitis. Proteins with significant differential expression, such as COL6A6, MPP1, GOLGA4, and SLC38A10, may play critical roles in the pathogenesis and progression of secondary atrophic rhinitis.