Abstract:Objective To investigate the role of lncRNA-mediated ceRNA network in pathophysiology of allergic rhinitis (AR) and its potential regulatory functions in programmed cell death(PCD).Methods AR patients and normal subjects were included. Their whole blood samples were obtained for RNA sequencing analysis, and differentially expressed lncRNA, miRNA and mRNA were screened to construct a lncRNA-miRNA-mRNA regulatory network based on ceRNA theory. GO and KEGG enrichment analyses were employed to predict the potential biological functions of mRNAs in the ceRNA network. Subsequently, the differentially expressed mRNAs interacted with PCD related genes, and a new ceRNA network was constructed, and target organ localization network analysis was performed on mRNAs in the network.Results Based on bioinformatics analysis, a total of 5 lncRNAs, 4 miRNAs and 46 mRNAs were extracted, and the ceRNA network of lncRNA-mediated up-regulation and down-regulation in AR was constructed. Functional enrichment analysis showed that it was significantly enriched in “calcium signaling pathway”, “PI3K/AKT signaling pathway” and “ERK1 and ERK2 cascade”. Next, a PCD related ceRNA regulatory network for two lncRNAs, AC008394.1 and HCP5, was constructed. In the target organ localization analysis of mRNAs in the ceRNA network, it was found that they were mostly expressed in neuroimmune-related organs.Conclusion A new ceRNA network in AR is successfully constructed and preliminarily exploration reveals that HCP5-mediated ceRNA network may play a role in AR by affecting apoptosis, which provides new insights and potential research options for the study of AR pathogenesis.
