MMP-2和MMP-9及胃蛋白酶对喉鳞状细胞癌侵袭作用的机制研究

Mechanism of MMP-2, MMP-9 and pepsin on the invasion of laryngeal squamous cell carcinoma

摘要:

目的探讨基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)及胃蛋白酶(Pepsin)对喉鳞状细胞癌局部侵袭的作用。方法收集2020年10月—2022年3月柳州市人民医院耳鼻咽喉科住院手术并经病理确诊的32例喉鳞状细胞癌患者的癌组织及其癌旁组织新鲜标本，通过实时荧光定量PCR(qRT-PCR)分别检测MMP-2、MMP-9及Pepsin mRNA在上述组织标本中的表达情况，结合临床资料分析。结果喉癌组织中MMP-2、MMP-9及Pepsin mRNA表达高于癌旁组织，差异具有统计学意义(P＜0.05)；Ⅲ、Ⅳ期组表达水平高于Ⅰ、Ⅱ期组(P＜0.05)，淋巴结转移组高于无转移组(P＜0.05)；MMP-2、MMP-9 mRNA在中-低分化鳞状细胞癌组的表达高于高分化组(P＜0.05)；Pepsin mRNA在60岁及以上喉癌组中表达高于60岁以下组(P＜0.05)。结论MMP-2、MMP-9和Pepsin在喉鳞状细胞癌的局部侵袭过程中发挥协同促进作用，预防和治疗咽喉反流是防止喉癌局部侵袭的重要环节。

Abstract:

ObjectiveTo investigate the effect of matrix metalloproteinase-2 (MMP-2), MMP-9 and pepsin on local invasion of laryngeal squamous cell carcinoma (LSCC).MethodsSpecimens of cancer tissue and paracancerous tissue from 32 patients with LSCC surgically treated in our department between Oct 2020 and March 2022 were collected. The mRNA expressions of MMP-2, MMP-9 and pepsin in the tissue samples were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and analyzed in combination with clinical data.ResultsThe mRNA expressions of MMP-2, MMP-9 and pepsin in LSCC tissues were higher than those in the adjacent tissues (P < 0.05). The expression levels in stage Ⅲ and Ⅳ groups were higher than those in stage Ⅰ and Ⅱ groups (P < 0.05), and that in lymph node metastasis group was higher than that in non-metastasis group (P < 0.05). The mRNA expressions of MMP-2 and MMP-9 in medium-low differentiated LSCC group were higher than those in well differentiated group (P < 0.05). The mRNA expression of pepsin in LSCC group with age ≥ 60 years was higher than that in LSCC group with age﹤60 years (P < 0.05).ConclusionMMP-2, MMP-9 and pepsin have synergistic effect on promoting the local invasion of LSCC. Prevention and treatment of laryngopharyngeal reflux is important to prevent local invasion of LSCC.