小鼠鼻腔灌洗及鼻粘膜获取方法的改进
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广州市科技计划项目市校(院)联合资助项目(202102010104),广东省自然科学基金面上项目(2021A1515012551),国家自然科学基金面上项目(82171764)


Improved method to obtain nasal lavage fluid and mucosa from mice
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    摘要:

    摘 要: 目的 小鼠变应性鼻炎模型在变应性鼻炎发生发展及治疗手段疗效评估的研究中具有重要的作用,然而小鼠鼻腔灌洗以及鼻粘膜组织取材因难度较大限制了该模型的应用。本研究在现有方法的基础上对小鼠鼻腔灌洗以及鼻粘膜的获取方法进行了改进,以提高小鼠鼻腔灌洗以及鼻粘膜获取的效率与质量。方法 本研究提出了一种简易的经后鼻道的鼻腔灌洗方法,并将它与经气管插管的鼻腔灌洗进行了比较,通过比较灌洗液回收率以及操作耗时,以确定两种方法的优劣。另外本研究还提出了两种小鼠鼻粘膜获取方法:即从鼻骨和上颚两个开放的窗口获取鼻粘膜,以及分部位获取鼻粘膜的方法,并通过图片的形式对过程予以了展示。结果 经过简单培训的实验员用后鼻道鼻腔灌洗法收集的灌洗液量要显著高于颇有经验的实验员用气管插管鼻腔灌洗法获取的灌洗液量,平均回收率达93%,而且操作耗时明显缩短。在小鼠鼻粘膜获取过程中本研究提出的两种方法,可以分别实现最大化的获取小鼠鼻粘膜,或分部位获取不同位置的鼻粘膜。结论 本研究提出的经后鼻道的鼻腔灌洗以及两种小鼠鼻粘膜获取方法,操作简单,效率高,容易掌握,将会为小鼠变应性鼻炎模型研究的取材方法提供重要参考。

    Abstract:

    【Abstract】 Objective The mouse model of allergic rhinitis plays an important role in the research on the occurrence and development of allergic rhinitis and the evaluation of the therapeutic effect. However, the difficulty in obtaining samples from nasal lavage and nasal mucosa in mice limits the application of this model. In this study, we improved the method to obtain nasal lavage and nasal mucosa from mice. Methods We proposed an easy method to obtain the nasal lavage of mouse through the postnasal way and compared it with the method usually used through tracheal way. In addition, we proposed two methods for obtaining the nasal mucosa of mice and demonstrated the process in the form of pictures. Results The recovery of lavage fluid collected by the simple-trained experimenter using the postnasal way was significantly higher than that obtained by the experienced experimenter using the tracheal way. and the average recovery rate of nasal lavage fluid was up to 93% in this new method, and the operation time is significantly shortened. In the process of obtaining the nasal mucosa of mice, the two methods proposed in this study can respectively achieve the maximum obtaining of the nasal mucosa of mice or obtain the nasal mucosa from different locations. Conclusion The methods to obtain nasal lavage and the nasal mucosa of mice proposed in this study are simple to operate, efficient and easy to grasp, and will provide an important reference for the study of the mouse model of allergic rhinitis.

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  • 收稿日期:2022-01-28
  • 最后修改日期:2022-03-09
  • 录用日期:2022-03-15
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