昆明小鼠耳蜗内脂肪酸转运蛋白4的表达与分布

Expression and distribution of fatty acid transporter 4 in Kunming mice cochlea

摘要:

目的 探索昆明小鼠耳蜗组织中脂肪酸转运蛋白4 (FATP4)的表达与分布。方法 选取健康成年(10周)昆明小鼠8只(16耳)，其中2只(4耳)用于免疫荧光染色，6只(12耳)用于Western blot蛋白检测。通过耳蜗石蜡切片免疫荧光染色检测FATP4在耳蜗内各组织中的表达分布，Western blot蛋白检测耳蜗组织FATP4的表达水平。结果 耳蜗石蜡切片免疫荧光染色提示FATP4主要分布于耳蜗螺旋神经节、蜗轴、支持细胞、内毛细胞、外毛细胞、血管纹的中间细胞，Western blot蛋白检测结果进一步验证了FATP4在昆明小鼠耳蜗表达。结论 本研究初步揭示了FATP4在成年昆明小鼠耳蜗中的表达具有一定的特异性，为进一步研究耳蜗内脂肪酸代谢提供了理论依据。

关键词:耳蜗;昆明小鼠;脂肪酸转运蛋白4;Hensen细胞

Abstract:

Objective To explore the expression and distribution of fatty acid transporter 4 (FATP4) in the cochlea of Kunming mice.Methods Eight healthy adult (10 weeks) Kunming mice (16 ears) were used, including 2 mice (4 ears) for immunofluorescence staining and 6 mice (12 ears) for Western blot. The expression and distribution of FATP4 in various tissues of the cochlea were detected by immunofluorescence staining of the paraffin section of the cochlea，and Western blot was used to observe the expression level of FATP4 in the cochlea.Results Immunofluorescence staining indicated that FATP4 was mainly distributed in spiral ganglion, modiolus, supporting cells, inner and outer hair cells as well as intermediate cells of stria vascularis. Western blot results further verified the expression of FATP4 in the cochlea of Kunming mice.Conclusion The distribution of FATP4 in the cochlea of adult Kunming mice possesses tissue specificity, which provides a theoretical basis for further research of fatty acid metabolism in the cochlea.

Key words:Cochlea;Kunming mouse;FATP4;Hensen cell