小鼠上下气道一致的变应性气道炎症动物模型的建立张勇12 张婷2 王志颐2 吴明海2 季俊峰2 陈仁杰3 陈伟2
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南京医科大学第四附属医院

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中国博士后基金面上项目(2017M623435);中国博士后科学基金特别资助项目(2018T111165)


The establishment of an animal model of allergic airway inflammation with consistent upper and lower airways in mice
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The Fourth Affiliated Hospital of Nanjing Medical University

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    目的: 建立小鼠上下气道一致的变应性气道炎症模型。方法: 16只雌性Balb/c小鼠随机分为模型组(8只)和对照组(8只),通过腹腔注射卵白蛋白(ovalbumin,OVA)作基础致敏,滴鼻与雾化吸入OVA续贯激发,末次激发后评价小鼠模型的症状、特异性IgE(specific immunoglobulin E,sIgE)浓度及鼻肺黏膜的病理改变(根据黏膜纤毛缺失程度,将上皮细胞状态分为4级(0-3级))。结果: 模型组鼻、肺部症状评分与对照组相比均有显著统计学差异(P?0.01)。模型组鼻、肺部黏膜破坏程度(1、2级)与对照组相比均有显著统计学差异(P?0.01),而3级均无统计学意义(P?0.05)。模型组鼻、肺灌洗及血清中OVAsIgE浓度均较对照组明显增高(P?0.01)。模型组鼻、肺部EOS和杯状细胞均较对照组明显增高(P?0.01)。模型组鼻、肺EOS数目呈显著正相关(r=0.775, P?0.01),杯状细胞数也呈正相关(r=0.723, P?0.05)。结论:本造模方法能够成功建立小鼠症状学、免疫学及病理学三方面上下气道一致的AAI模型。

    Abstract:

    Objective: Establish an animal model of allergic airway inflammation(AAI) with consistent airway in the upper and lower airways of mice. Method: OVA-sensitized 16 BALB/c mice were randomly divided into model group (8 animals) and control group (8 animals) ,intranasal OVA exposure three times a week for 3 weeks, followed by 5 days of OVA aerosol challenge, and the symptoms, specific IgE concentration and pathological changes of nasal lung mucosa were evaluated in the mice model after the last challenge(Epithelial cell states were classified into 4 levels (0-3) according to the degree of mucosal cilium loss). Result: The nasal and pulmonary symptom scores of the model group were significantly higher than those of the ctrl group(P?0.01). There was a statistically significant difference of nasal and pulmonary mucosal damage (grades 1, 2) of the model group compared with the ctrl group(P?0.01), but there was no statistical significance at grade 3(P?0.05).Nasal lavage, lung lavage and serum OVAsIgE concentrations in the model group were significantly higher than those in the ctrl group(P?0.01). The nasal and pulmonary EOS and GC in the model group were significantly higher than those in the ctrl group (P?0.01).There was a significant positive correlation between nasal and pulmonary EOS in the model group (r=0.775, P?0.01), and the number of GC was also positively correlated (r=0.723, P?0.05).Conclusion: The modeling method can successfully establish AAI model with upper and lower airway consistency in three aspects of symptom, immunology and pathology in mice.

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  • 收稿日期:2022-01-05
  • 最后修改日期:2022-03-15
  • 录用日期:2022-03-17
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