Abstract:Objective: Establish an animal model of allergic airway inflammation(AAI) with consistent airway in the upper and lower airways of mice. Method: OVA-sensitized 16 BALB/c mice were randomly divided into model group (8 animals) and control group (8 animals) ,intranasal OVA exposure three times a week for 3 weeks, followed by 5 days of OVA aerosol challenge, and the symptoms, specific IgE concentration and pathological changes of nasal lung mucosa were evaluated in the mice model after the last challenge(Epithelial cell states were classified into 4 levels (0-3) according to the degree of mucosal cilium loss). Result: The nasal and pulmonary symptom scores of the model group were significantly higher than those of the ctrl group(P?0.01). There was a statistically significant difference of nasal and pulmonary mucosal damage (grades 1, 2) of the model group compared with the ctrl group(P?0.01), but there was no statistical significance at grade 3(P?0.05).Nasal lavage, lung lavage and serum OVAsIgE concentrations in the model group were significantly higher than those in the ctrl group(P?0.01). The nasal and pulmonary EOS and GC in the model group were significantly higher than those in the ctrl group (P?0.01).There was a significant positive correlation between nasal and pulmonary EOS in the model group (r=0.775, P?0.01), and the number of GC was also positively correlated (r=0.723, P?0.05).Conclusion: The modeling method can successfully establish AAI model with upper and lower airway consistency in three aspects of symptom, immunology and pathology in mice.