鼻咽癌组织中BLU与cyclin D1和cyclin B1的表达及其临床意义

Expressions of BLU, cyclin D1 and cyclin B1 and their clinical significances in nasopharyngeal carcinoma

摘要:

目的 探究BLU与cyclin D1和cyclin B1在鼻咽癌（NPC）组织中的表达及其临床意义。方法 采用免疫组化法检测97例NPC组织及36例慢性鼻咽炎（CN）组织中BLU与cyclin D1、cyclin B1的表达，运用统计学方法分析三者的表达与NPC患者临床病理特征参数及预后的关系。结果 在NPC组织中，BLU阳性表达率低于CN中的阳性表达率，cyclin D1与cyclin B1阳性表达率高于CN组织的阳性表达率（P<0.05）。BLU表达与年龄、T分期有相关性，cyclin D1表达与临床分期、淋巴结转移有相关性，cyclin B1表达与临床分期与T分期有相关性（P<0.05）。Spearman等级相关分析结果显示BLU与cyclin D1在NPC组织中表达呈负相关（r=-0.378，P=0.011），BLU与cyclin B1在NPC组织中表达无明显相关性（r=0.096，P=0.089），cyclin D1与cyclin B1在NPC组织中表达无相关性（r=0.084，P=0.191）。BLU表达阳性组的总生存率高于表达阴性组，cyclin D1表达阳性组总生存率低于表达阴性组（P<0.05），cyclin B1表达阳性组与表达阴性组总生存率差异无统计学意义（P>0.05）。Log-rank法分析结果显示BLU、cyclin D1、T分期、N分期与患者的预后有关（P<0.05）；Cox回归模型分析显示BLU、cyclin D1及淋巴结转移是NPC预后的独立预测因子。结论 BLU表达下调，而cyclin B1和cyclin D1高表达，可能在NPC发生发展中产生重要作用，其有可能成为其中评估NPC预后的标志物。

Abstract:

Objective To explore the expressions of BLU, cyclin D1 and cyclin B1 and their clinical significances in nasopharyngeal carcinoma (NPC).Methods Immunohistochemical method was used to evaluate the expressions of BLU, cyclin D1 and cyclin B1 in 97 NPC tissues and 36 chronic nasopharyngitis tissues, and the relationships between the expressions of these three proteins and clinicopathological characteristic parameters as well as the prognosis of NPC patients were analyzed.Results The positive rate of BLU in NPC was lower than that in chronic nasopharyngitis, while the positive rates of cyclin D1 and cyclin B1 in NPC were higher than those in chronic nasopharyngitis (all P<0.05). BLU expression was correlated with patients’ age and T stage of tumor, while cyclin D1 expression was correlated with clinical stage and lymph node metastasis, and cyclin B1 expression was correlated with clinical stage and T stage (all P<0.05). Spearman rank correlation analysis showed that BLU was negatively correlated with cyclin D1 (r=-0.378, P=0.011) but not significantly correlated with cyclin B1 in NPC tissues (r=0.096, P=0.089), and cyclin D1 and cyclin B1 were not significantly correlated in NPC tissues (r=0.084, P=0.191). The overall survival rate of the BLU positive NPC group was higher than that of the negative group, and the overall survival rate of the cyclin D1 positive NPC group was lower than that of the negative group (P<0.05). In addition, the difference of the overall survival rate between the cyclin B1 positive group and the negative group was statistically insignificant (P>0.05). The log-rank analysis indicated that BLU, cyclin D1 and T stage as well as N stage were related to the prognosis of the NPC patients (all P<0.05). Cox regression model analysis showed that BLU, cyclin D1 and lymph node metastasis were independent predictors in the prognosis.Conclusion The down-regulated expression of BLU and up-regulated expressions of cyclin B1 and cyclin D1 may play important roles in the occurrence and development of NPC. Therefor, they may be used as markers to evaluate the prognosis of NPC patients.