变应性鼻炎血清sPD-L1表达与T淋巴细胞亚群的关系

Relationship between serum sPD-L1 expression and T lymphocyte subsets in allergic rhinitis

摘要:

目的 探讨变应性鼻炎（AR）血清sPD-L1表达与T淋巴细胞亚群的关系。方法 选取2018年6月—2020年2月收治的AR患者120例，并纳入同期体检健康者50例作为对照组。采用Elisa法测定两组患者血清sPD-L1水平，采用流式细胞技术检查CD3⁺、CD8⁺、CD4⁺和CD4⁺/CD8⁺水平；采用Spearman分析sPD-L1与外周血CD3⁺、CD4⁺、CD8⁺和CD4⁺/CD8⁺的关系。结果 研究组sPD-L1水平和CD4⁺/CD8⁺水平显著高于对照组（P<0.05）；研究组CD3⁺、CD4⁺和CD8⁺水平显著低于对照组（P<0.05）；血清sPD-L1水平与CD3⁺、CD4⁺、CD8⁺呈显著负相关（r₁=-0.754，r₂=-0.817，r₃=-0.691，P<0.05），而与CD4⁺/CD8⁺呈正相关（r=0.810，P<0.05）。结论 AR患者血清sPD-L1表达上调与T淋巴细胞亚群的紊乱密切相关。

Abstract:

Objective To investigate the relationship between serum sPD-L1 expression and T lymphocyte subsets in allergic rhinitis (AR).Methods A total of 120 patients with AR admitted to our hospital from June 2018 to Feb 2020 were selected. In addition, 50 healthy physical examinees during the same period were included as the control group. ELISA method was used to determine the serum sPD-L1 levels and flow cytometry to check the CD3⁺, CD8⁺, CD4⁺ and CD4⁺/CD8⁺ levels in the both groups. Spearman was used to analyze the relationships between sPD-L1 and peripheral blood levels of CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺.Results The levels of sPD-L1 and CD4⁺/CD8⁺ in the AR group were significantly higher than those in the control group (all P<0.05). The levels of CD3⁺, CD4⁺, and CD8⁺ in the AR group were significantly lower than those in the control group (all P<0.05). Serum sPD-L1 level was negatively correlated with the levels of CD3⁺, CD4⁺, CD8⁺ (r₁=-0.754, r₂=-0.817, r₃=-0.691, P<0.05), and positively with CD4⁺/CD8⁺ (r=0.810, P<0.05).Conclusion The up-regulation of serum sPD-L1 expression in AR patients is closely related to the disorder of T lymphocyte subsets.