TRPC6与OMP在慢性鼻鼻窦炎患者嗅上皮中的表达及与嗅觉功能相关性分析

Expression of TRPC6 and OMP in the olfactory epithelium and its correlation with olfactory function in patients with chronic rhinosinusitis

摘要:

目的 研究瞬时受体电位通道6（TRPC6）与嗅觉标记蛋白（OMP）在慢性鼻-鼻窦炎（CRS）引起的嗅觉减退患者嗅上皮中的表达以及与嗅觉减退的关系。方法 收集20例CRS伴嗅觉减退的患者为实验组，10例鼻中隔偏曲且嗅觉正常患者为对照组。T&T嗅觉计测试检测两组患者的嗅觉，取手术中切除的嗅区黏膜作为研究对象。免疫组化和蛋白印迹分析（Western Blot）检测TRPC6与OMP在嗅上皮中的表达，并比较CRS伴不同程度嗅觉功能减退患者嗅上皮中TRPC6与OMP的表达水平，同时分析TRPC6与OMP表达量与嗅觉功能相关性。结果 实验组嗅觉T&T评分明显高于对照组，表明入组的CRS患者存在嗅觉功能减退（P<0.001）。免疫组化和Western Blot结果表明：与对照组相比，实验组TRPC6与OMP阳性细胞数和蛋白表达量明显减少（P<0.001）。此外，T&T评估得分与嗅上皮组织中TRPC6和OMP表达水平呈现负相关（TRPC6， R²=0.91， P<0.001；OMP：R²=0.853 8， P<0.001）。结论 TRPC6在CRS引起的嗅觉减退患者的嗅上皮中表达下调，且嗅觉功能减退程度与TRPC6含量呈负相关性，OMP作为嗅觉的客观反应，同样表达下调，故我们推断TRPC6的表达下调可能是导致CRS引起嗅觉减退的生物学机制之一。

Abstract:

Objective To study the expressions of transient receptor potential canonical 6 (TRPC6) and OMP (Olfactory marker protein, OMP) in the olfactory epithelium of patients with hyposmia caused by chronic rhinosinusitis (CRS) and their relationship with hyposmia. Methods Twenty patients with CRS with hyposmia were enrolled as the experimental group. Ten patients with deviated nasal septum and normal olfactory sense were enrolled as the control group. T&T olfactometer test was used to evaluate the olfactory function in both groups. Immunohistochemistry and Western Blot were used to detect and analyze the expressions of TRPC6 and OMP in the olfactory epithelium of patients with CRS with different degrees of olfactory function. At the same time, the correlation between the expressions of TRPC6 and OMP and the olfactory function was analyzed. Results The olfactory T&T score in the test group was significantly higher than that in control group, which indicated the patients with CRS generally had decreased the olfactory function (P<0.001). Immunohistochemical staining and Western blot showed that the positive cells and the expressions of TRPC6 and OMP were significantly reduced in the test group than those in the control group. In addition, the T&T assessment score was negatively correlated with the expression levels of TRPC6 and OMP in the olfactory epithelial tissue (TRPC6, R²=0.91, P<0.001; OMP:R²=0.8538, P<0.001). Conclusion The expression of TRPC6 was down-regulated in the olfactory epithelium of patients with hyposmia caused by CRS, and the degree of hypo-olfactory function was negatively correlated with the level of TRPC6. As an objective response of olfaction, OMP was also down-regulated. Therefore, we speculated that the down-regulation of TRPC6 expression might be one of the biological mechanisms of hyposmia caused by CRS.