CD4+ Treg细胞对变应性鼻炎患者外周血单个核细胞的作用

Influences of CD4+ Tregs on peripheral blood mononuclear cells from allergic rhinitis patients

摘要:

目的 探讨CD4⁺ Treg细胞对变应性鼻炎（AR）患者外周血单个核细胞（PBMCs）的作用。方法 招募正常人（对照组）和AR患者（AR组）各15例，分别从两组志愿者外周血中获取PBMCs，接着从PBMCs中分离、提纯CD4⁺ Treg细胞，检测该细胞在总CD4⁺ T细胞中所占的百分比，并将其进行体外培养，然后检测CD4⁺ Treg细胞培养基中白介素（IL）-10和转化生长因子（TGF）-β及其mRNA的含量，最后，将体外培养的CD4⁺ Treg细胞加入到AR患者的PBMCs培养基中进行干预，并检测培养基中IL-4和IL-5的浓度。结果 AR患者CD4⁺Treg细胞（2.523±0.260）在总CD4⁺ T细胞中所占的百分比较对照组（4.716±0.390）减少，但AR组患者CD4⁺ Treg细胞培养基中IL-10（96.19±6.96 vs 33.69±3.88）和TGF-β（77.73±7.96 vs 31.39±3.15）及其mRNA（3.30±0.27 vs 0.26±0.05；3.29±0.26 vs 0.26±0.03）的含量却显著增多，AR组PBMCs培养基中IL-4（260.40±12.49）和IL-5（287.10±18.52）的浓度较对照组（23.07±3.26；19.62±2.46）为高，径AR患者的CD4⁺ Treg细胞干预后，AR患者PBMCs培养基中IL-4（120.60±9.58）和IL-5（137.60±12.33）的浓度显著降低，但对照组CD4⁺ Treg细胞的干预没有改变培养基中这些细胞因子（241.70±12.44；262.40±18.88）的含量。结论 CD4⁺ Treg细胞在变应性炎症状态下可以激活并起到抑制炎症的作用。

Abstract:

Objective The present study was aimed to assess the influences of CD4⁺ Tregs on peripheral blood mononuclear cells (PBMCs) from AR patients. Methods Fifteen normal subjects (normal group) and 15 AR patients (AR group) were enrolled. Their PBMCs were obtained, and CD4⁺ Tregs were separated and purified from PBMCs with calculation of the percentage of CD4⁺ Tregs in total CD4⁺ T cells and then cultured in vitro. The levels of interleukin (IL)-10 and transforming growth factor (TGF)-β and their mRNAs in Tregs cultures were detected. Finally, the in vitro cultured CD4⁺ Tregs were administered into PBMCs cultures from AR patients and the concentrations of IL-4 and IL-5 in the cultures were measured. Results The percentage of CD4⁺ Tregs in the total CD4⁺ T cells from PBMCs of AR group (2.523±0.26) was lower than that of the normal group (4.716±0.39). However, the contents of IL-10 (96.19±6.96 vs 33.69±3.88) and TGF-β (77.73±7.96 vs 31.39±3.15) as well as their mRNAs (3.30±0.27 vs 0.26±0.05; 3.29±0.26 vs 0.26±0.03) in CD4⁺ Tregs cultures of the AR group were higher than those of the normal group. The concentrations of IL-4 and IL-5 in PBMCs cultures of the AR group (260.40±12.49; 287.10±18.52) were higher than those of the normal group (23.07±3.26; 19.62±2.46), and decreased after the administration of CD4⁺ Tregs from AR patients (120.60±9.58; 137.60±12.33). The treatment of CD4⁺ Tregs from normal subjects did not change concentrations of IL-4 (241.70±12.44) and IL-5 (262.40±18.88) in PBMCs cultures of the AR patients. Conclusion The CD4⁺ Tregs may be activated in allergic condition and alleviate inflammatory responses in AR.