SEMA3D在喉癌组织中的表达及临床意义

The expression of SEMA3D and its clinical significance in human laryngeal cancer

摘要:

目的探讨喉癌组织中轴突导向因子3D(SEMA3D)的表达与患者临床病理特征和预后的关系。方法收集2015年1月—2016年12月经外科手术切除的喉癌组织及对应癌旁组织标本61例，应用免疫组化SP法检测SEMA3D的表达情况，并对SEMA3D与喉癌临床病理特征及预后的关系进行统计学分析。结果免疫组化SP法显示，SEMA3D在喉癌组织中的表达水平显著低于在癌旁正常组织中的表达水平（P<0.05）。SEMA3D表达与患者的临床分期和颈部淋巴结转移密切相关(P<0.05)。KaplanMeier生存分析显示，SEMA3D表达与患者生存率具有相关性（P＜0.001）。单变量Logrank检验分析显示吸烟、临床分期、颈部淋巴结转移和SEAM3D表达与患者预后具有相关性，进一步行Cox多因素回归分析发现SEMA3D低表达是影响喉癌预后的独立危险因素。结论SEMA3D蛋白低表达是影响喉癌预后的独立危险因素。SEMA3D蛋白阳性表达的喉癌患者预后较好，有望成为喉癌患者预后评估的新的生物标志物。

Abstract:

ObjectiveTo investigate the expression of Semaphporin 3D in laryngeal cancer tissue and its relationship with clinicopathological characteristics and prognosis of patients.MethodsSixtyone laryngeal cancer tissues and normal tissues adjacent to carcinoma were obtained from January 2015 to December 2016. Immunohistochemistry SP staining was used to detect the expression of Semaphporin 3D. The relationships of its expression with clinicopathological characteristics and prognosis were analyzed.ResultsImmunohistochemistry SP method showed that SEMA3D expression was significantly lower in laryngeal cancer tissue than in normal tissue adjacent to carcinoma(P＜0．05). The expression of SEMA3D was closely related to clinical stage and lymph node metastasis(P＜0. 05). KaplanMeier survival analysis showed that SEMA3D expression was correlated with patient survival(P＜0.001). Univariate Logrank test showed that smoking, clincial staging, lymphnode metastasis and expression of SEMA3D were associated with the prognosis of patients. Multivariate Coxregression analysis further showed that the low expression of SEMA3D was an independent risk factor affecting the prognosis of laryngeal cancer.ConclusionsLow expression of SEMA3D protein is an independent risk factor for the prognosis of laryngeal cancer. Laryngeal cancer patients with positive SEMA3D expression show better prognosis than those with negative SEMA3D expression, which indicates that SEMA3D may be a potential biomarker for the prediction of prognosis in patients with laryngeal cancer.