CDR1as在喉鳞状细胞癌中的表达及临床意义

Expression and clinic significance of CDR1as in laryngeal squamous cell carcinoma

摘要:

目的探讨环状RNA ciR7(CDR1as)在喉鳞状细胞癌（laryngeal squamous cell carcinoma，LSCC）中的表达及临床意义，并分析CDR1as与miR7的相关性。方法收集2011—2013年手术切除并病理确诊为鳞状细胞癌的标本61例，同期选取病理确诊为炎症或正常黏膜的癌旁组织（距肿瘤切缘大于0.5 cm）标本48例，采用实时荧光定量PCR方法检测喉癌及癌旁组织中CDR1as、miR7的转录表达水平。结果Realtime PCR显示，在LSCC组织中，CDR1as mRNA表达水平均显著高于癌旁组织（P＜0.001）;miR7 mRNA表达水平均显著低于癌旁组织（P＜0.0001）; CDR1as在淋巴结转移患者的LSCC组织中表达水平较无淋巴结转移患者的LSCC组织中显著增高(P＜0.01)；CDR1as在不同TNM分期患者的喉癌组织中表达水平差异具有统计学意义(P＜0.05)；CDR1as在LSCC组织中表达水平与患者的预后相关 (P=0.02)；同时，CDR1as表达水平与miR7表达呈负性相关(r=-0.643，P=0．001)。结论CDR1as参与LSCC的发生和发展，其表达可以作为判断预后的一项指标，CDR1as与miR7呈现出的一定的相关性，CDR1as可能通过靶向调控miR7的表达影响喉癌进展。

Abstract:

Abstract:ObjectiveTo investigate the expression and clinical significance of ciRNA ciR7 (CDR1as) in laryngeal squamous cell carcinoma (LSCC), and to analyze the correlation between CDR1as and miR7.MethodsFrom 2011 to 2013,realtime fluorescence quantitative polymerase chain reaction (RTPCR) was used to detect the transcriptional expression levels of CDR1as and miR7 in 61 specimens of laryngeal squamous cell carcinoma and 48 of normal tissues adjacent to tumor.ResultsRTPCR showed that the expression of CDR1as in the LSCC tissues was significantly higher than that in the adjacent tissues (P<0.001), the expression of miR7 in the LSCC tissues was significantly lower than that in the adjacent tissues (P<0.0001), the expression of CDR1as in LSCC tissues from patients with lymph node metastasis was significantly higher than that from those without lymph node metastasis(P<0.01), and the expression level of CDR1as in the LSCC tissues of patients with different TNM stages were significantly different (P<0.05). The difference of 5year survival rate between patients with high expression level of CDR1as in the LSCC tissues and those with low expression level was statistically significant (P=0.02), meanwhile, the expression level of CDR1as was negatively correlated with that of miR7 (r=-0.643, P=0.001).ConclusionsCDR1as is involved in the occurrence and development of LSCC, and its expression can be used as a prognostic indicator of this tumor. CDR1as has a certain correlation with miR7. CDR1as may affect the progression of LSCC by targetedregulating the expression of miR7.