自噬标志基因Beclin1和p62在变应性鼻炎中的表达及临床意义

Expression and clinical significance of autophagy marker genes Beclin1 and p62 in allergic rhinitis

摘要:

目的研究自噬标志基因Beclin1和p62在变应性鼻炎（allergic rhinitis,AR）患者鼻甲黏膜组织的表达及临床意义。方法应用实时荧光定量PCR技术检测Beclin1和p62两个基因在68例AR患者及5例健康对照者中的表达水平，并对照2年随访期内诊断为慢性鼻-鼻窦炎鼻息肉（CRSwNP）的患者，分析两个基因的表达水平在后期是否发展为CRSwNP的AR患者不同个体中的差异。结果68例AR患者在2年之内有11例发展为CRSwNP，发生率为16.18%，其Beclin1基因表达平均水平值为0.38±0.13，显著低于未发展为CRSwNP的另外57例患者Beclin1基因表达平均水平值0.79±0.23，差异具有统计学意义（P<0.01），而且此11例患者在AR阶段的p62基因表达平均水平值为2.67±0.55，显著高于未发展为CRSwNP的另外57例患者p62基因表达平均值1.39±0.32，差异具有统计学意义（P<0.01）。结论自噬过程的两个标志基因Beclin1的降低和p62的升高将降低自噬水平，AR患者在随访期间发展为CRSwNP,与已证实的CRSwNP患者中Beclin1基因表达降低、p62基因表达升高、自噬水平降低等研究结果一致，所以临床诊断上AR患者中Beclin1和p62基因的表达水平变化可以为预判其后期是否发展为CRSwNP风险提供临床参考。

Abstract:

ObjectiveTo research the expression and clinical significance of two autophagy marker genes Beclin1 and p62 in allergic rhinitis (AR) patients.MethodsQuantitative realtime polymerase chain reaction (qRTPCR) was adopted to detect the expression levels of Beclin1 and p62 in nasal mucosal specimens from 68 AR patients and 5 healthy controls respectively. All the 73 subjects had been followed up for two years to observe whether chronic rhinosinusitis with nasal polypsis (CRSwNP) developed. Then the expression differences of the two genes between the AR patients who did or didn’t develop into CRSwNP were analyzed.Results11 of the 68 AR patients developed into CRSwNP with an incidence of 16.18%. The average expression level of Beclin1 gene of the 11 patients was 0.38±0.13, which was significantly lower than that of the 57 AR patients without CRSwNP 0.79±0.23. On the other hand, the average expression level of p62 gene of the 11 patients 2.67±0.55 was significantly higher than that of the 57 AR patients without CRSwNP 1.39±0.32. Both the expression differences were statistically significant (both P<0.01).ConclusionsThe expression of Beclin1 gene is decreased and that of p62 gene is elevated in AR patients who developed into CRSwNP, which is consistent with the reported studies in CRSwNP patients. Therefore, the expression changes of Beclin1 and p62 in AR patients may be expected to predict the risk of CRSwNP in clinic.