黄花蒿花粉致敏的变应性鼻炎小鼠模型的建立

Establishment of a mouse model with allergic rhinitis sensitized by Artemisia pollen

摘要:

目的通过建立稳定的实验性变应性鼻炎(allergic rhinitis,AR)BALB/C 小鼠模型，成功建立黄花蒿花粉变应原致敏的AR小鼠模型。方法选取BALB/C雌性小鼠约20 g，共20只，随机分为AR组和溶媒组，每组各10只。第1、2周，每只AR组小鼠足底皮下注射 20 μl黄花蒿花粉抗原溶液，对小鼠进行初次致敏，溶媒组小鼠用等量抗原溶媒溶液处理，每周1次，连续2周;第3周，致敏方法同前2周，但剂量减半，同时AR组小鼠双侧鼻腔各滴入 10 μl黄花蒿花粉抗原溶液，溶媒组小鼠双侧鼻腔滴入等量抗原溶媒溶液，隔天1次;第4周，AR组小鼠双侧鼻腔各滴入 10 μl黄花蒿花粉抗原溶液，溶媒组小鼠双侧鼻腔滴入等量抗原溶媒溶液，每天1次。建立模型：模型建立时间不少于35 d。观察小鼠的临床症状，采用叠加量化积分法计分，行为学积分>5分者表示造模成功，随后通过小鼠鼻黏膜HE染色观察病理学改变。结果AR组小鼠经过鼻腔局部激发致敏后均出现AR的临床症状如鼻痒、流涕、打喷嚏等，而溶媒组小鼠均无上述症状。结论本实验建立的AR小鼠模型是成功的，操作简单、重复性好，为AR的免疫学研究提供了有效的方法及组织形态学依据，值得推广。

Abstract:

Abstract:ObjectiveTo establish a mouse model with allergic rhinitis (AR) sensitized by pollen allergen of Artemisia annua.Methods20 BALB/C female mice were randomly divided into model group (AR group) and control group (solvent group) with 10 mice in each group.In the first and second week,20μl of Artemisia annua pollen antigen solution was subcutaneously injected into the sole of each AR group, and the mice were first sensitized.in that first and second week,20μl of artemisia annua pollen antigen solution was subcutaneously injected into the sole of each AR group, and the mice were first sensitized, and the vehicle group mice were treated with an equal amount of an antigen solvent solution, once a week for 2 weeks;In the third week, the sensitization method was the same as that in the previous two weeks, but the dose was reduced by half. At the same time, the twosided nasal cavity of the AR group was dropped to 10μl of the pollen antigen solution of the Artemisia annua L., the twoside nasal cavity of the vehicle group was dropped into the equivalent antigen solvent solution, and the other day was 1 time;In the fourth week, the twosided nasal cavity of the AR group was dropped to 10μl of Artemisia annua pollen antigen solution, and the twoside nasal cavity of the vehicle group was dropped into the equivalent antigen solvent solution and once a day. Set up the model,the model building time is not less than 35 d.The clinical symptoms of mice were observed. The behavioral score obtained by superimposed quantitative integration >5 indicated successful establishment of AR model, and then the pathological changes were observed by HE staining of nasal mucosae.ResultsAll the mice in the AR group developed AR symptoms such as itching, runny nose and sneezing after local sensitization of nasal cavity.ConclusionWith advantages of simple operation and good reproducibility, the successful establishment of mouse model of AR in this experiment is worth popularizing and provides an effective method and histomorphological basis for the immunological study of AR.