槲皮素对大鼠变应性鼻炎血清Th1/Th2细胞因子表达的影响

Effects of quercetin on expressions of Th1/Th2 cytokines in serum of experimental allergic rhinitis in rats

摘要:

目的分析槲皮素（QUE）对大鼠变应性鼻炎（allergic rhinitis， AR）血清T细胞辅助因子Th1/Th2表达的影响。方法采用二异氰酸甲苯酯（TDI）制作大鼠实验性AR模型并进行评分，45只大鼠按照数字随机法平分为3组：正常对照组、模型组和实验组，正常对照组为正常大鼠，模型组和实验组大鼠进行变应性鼻炎造模。实验组利用QUE 80 mg/kg腹腔注射治疗2周，而正常对照组和模型组均注射等量生理盐水。2周后HE染色观察各组大鼠鼻黏膜组织学变化；ELISA法检测血清中细胞因子白细胞介素-2（IL2）、干扰素-γ（IFNγ）以及IL4、IL5水平；实时荧光定量PCR（qPCR）检测基因Tim1、Tim3表达。结果2周后实验组评分显著低于正常对照组与模型组（P＜0.05）；ELISA检测显示模型组IL2、IFNγ显著低于正常对照组（P＜0.05），IL4，IL5显著高于正常对照组（P＜0.05）；实验组IL2、IFNγ显著高于模型组（P＜0.05），IL4，IL5显著低于模型组（P＜0.05）；qPCR检测显示实验组大鼠鼻黏膜组织Tim1、Tim3表达明显低于模型组（P＜0.05），模型组大鼠鼻黏膜组织Tim1、Tim3表达明显高于正常对照组（P＜0.05），实验组大鼠鼻黏膜组织Tim1、Tim3表达明显高于正常对照组（P＜0.05）。结论QUE能不同程度减轻大鼠变应性鼻炎鼻部症状，改善鼻黏膜炎症程度；通过调节Th1和Th2细胞因子表达，从而调节大鼠变应性鼻炎Th1/Th2细胞因子平衡，对变应性鼻炎具有一定保护作用。

关键词:变应性鼻炎|炎症反应|Th1/Th2|槲皮素|大鼠

Abstract:

ObjectiveTo explore the effects of quercetin on expressions of Th1/Th2 cytokines in serum of experimental allergic rhinitis (AR) in rats.Methods45 rats were equally divided into 3 groups, the control group, model group and experiment group. Rats in the control group received no treatment, while those in the model group and the experiment group were treated with toluene diisocyanate for model establishment of AR. Then, the rats in the experiment group were given intraperitoneal injection of quercetin (80mg/kg) for 2 weeks, and those in the control group and model group were treated with physiological saline equivalently. Histological changes of nasal mucosa were observed by HE staining. Besides, the levels of serum interleukin2 (IL2), interferonγ (IFNγ), IL4 and IL5 were evaluated by ELISA, and realtime fluorescent quantitative polymerase chain reaction (qRTPCR) was adopted to the expressions of Tim1 and Tim3.ResultsThe scores of nasal symptoms in the experiment group were significantly lower than those in the control group and the model group (both P＜0.05). ELISA showed that the serum levels of IL2 and IFNγ in model group were significantly lower than those in the control group (both P＜0.05), while the serum levels of IL4 and IL5 in model group were significantly higher than those in the control group (both P＜0.05). Compared with the model group, the serum levels of IL2 and IFNγ in the experiment group were increased, and those of IL4 and IL5 got decreased (all P＜0.05). qRTPCR revealed that the mRNA expressions of Tim1 and Tim3 in the experiment group were markedly lower than those in the model group, but higher than those in the control group (all P＜0.05). Meanwhile, their expressions in the model group were significantly higher than those in the control group (both P＜0.05).ConclusionQuercetin can improve nasal symptoms and relief mucosal inflammation in AR rats. It protects the nasal mucosa by regulating the expressions of Th1 and Th2 cytokines.

Key words:Allergic rhinitis|Quercetin|Th1/Th2| Inflammatory reaction|Rat