白细胞介素23受体基因多态性与变应性鼻炎易感性的相关性研究

Study on correlation between IL23R gene polymorphisms and susceptibility of allergic rhinitis

摘要:

 目的研究白细胞介素23受体（IL23R）基因多态性与中国河南地区汉族人群变应性鼻炎（allergic rhinitis，AR）发病易感性间的关系。方法收集114例河南地区汉族AR患者（AR组）及264例正常人群（对照组）全血样品进行研究。提取血液样本中的DNA，并运应用PCRSanger测序法对DNA样本中IL23R基因的多态位点rs7517847进行基因分型分析。结果114例AR组中AA、AC、CC基因型频率分别为23.7%、56.1%和20.2%；264例对照组中AA、AC和CC基因型频率为40.5%、45.8%和13.6%。AR组中rs7517847位点的等位基因AA、AC、CC分布与正常对照组比较差异有统计学意义（χ2=11.068，P=0.004）；等位基因为CC基因型的个体患AR的比例为40.0%（23/59），高于AA基因型（20.1%）和AC基因型（34.6%）的个体。A和C等位基因频率在AR组与正常对照组分布无显著性差异（χ2=0.256，P=0.187）。结论IL23R基因rs7517847位点的多态性与AR易感性有关；携带C等位基因且为CC基因型的个体患AR的风险性增大。

关键词: ;白细胞介素23受体|变应性鼻炎|基因检测|单核苷酸多态性|易感性

Abstract:

 ObjectiveTo investigate the relationship between susceptibility of allergic rhinitis (AR) and single nucleotide polymorphism (SNP) at rs7517847 in interleukin23 receptor (IL23R) in Henan Han population of China. Methods114 AR patients of Han nationality from Henan Province and 264 unrelated healthy individuals from the same geographic region were enrolled in this study. DNA from blood samples was extracted and the genotypes of IL23R polymorphisms (rs7517847) in DNA samples were genotyped using PCRSanger sequencing.ResultsAA, AC and CC genotype frequencies of 114 AR cases were 23.7%, 56.1% and 20.2%, respectively. AA, AC and CC genotype frequencies of the 264 controls were 40.5%, 45.8% and 13.6%, respectively. The difference of AA, AC and CC frequency distribution between the AR and control group was statistically significant (χ2=11.068，P=0.004). The AR ratio in individuals with CC genotype was 40.0% (23/59), which was higher than those with AA genotype (20.1%) and AC genotype (34.6%). Ther difference of A and C allele frequency distribution between the AR and control group was statistically insignificant (χ2=0.256，P=0.187). ConclusionPolymorphism of IL23R gene (rs7517847) is associated with susceptibility of AR in the Han Population in Henan Province. The risk of AR will be increased if the person carrying C allele and CC genotyoe.

Key words: ;Interleukin23R| Allergic rhinitis|Gene detection|Singlenucleotide polymorphism| Susceptibility