血浆长链非编码RNA NEAT1和H19及MALAT1在鼻咽癌中的表达及临床意义

Expressions and clinical significances of plasma long noncoding RNAs NEAT1, H19 and MALAT1 in nasopharyngeal carcinoma

摘要:

 目的探讨长链非编码RNA（long noncoding RNA, lncRNA）3个分子NEAT1和H19及MALAT1在鼻咽癌患者血浆中的表达水平及其在鼻咽癌诊断中的价值。方法收集91例病理确诊的鼻咽癌患者和100例健康体检者血浆作为研究对象。采用定量逆转录PCR检测上述3个lncRNA分子在鼻咽癌患者及健康者血浆中的表达水平。统计分析鼻咽癌患者血浆lncRNA NEAT1、H19和MALAT1 的表达水平与临床因素之间的关系，受试者工作特征（ROC）曲线和曲线下面积（AUC）评估血浆这3个lncRNA分子诊断鼻咽癌的效能。结果鼻咽癌患者血浆lncRNA NEAT1、H19和MALAT1的相对表达量均显著高于对照组（P<0.001）。MALAT1表达水平与N分期相关。血浆NEAT1、H19和MALAT1检测诊断鼻咽癌的AUC分别为0.715、0.708和0.704；NEAT1与H19联合，诊断鼻咽癌AUC增加至0.734。结论鼻咽癌患者血浆lncRNA NEAT1、H19和MALAT1有可能成为鼻咽癌辅助诊断的标志物。

Abstract:

 ObjectiveTo investigate the expressions of long noncoding RNAs (lncRNA) NEAT1, H19 and MALAT1 in plasma of nasopharyngeal carcinoma (NPC) patients and their values in the diagnosis of NPC.MethodsThe plasma samples were collected from 91 primary NPC patients and 100 healthy controls. The expression levels of NEAT1, H19 and MALAT1 were detected by quantitative realtime polymerase chain reaction (qTRPCR) in all samples. Associations between lncRNAs expressions and clinicopathological features of NPC were analyzed. The receiver operating characteristic curve (ROC) and the area under ROC (AUC) were used to evaluate the diagnostic performance of the lncRNAs for NPC.ResultsThe plasma levels of lncRNA NEAT1, H19 and MALAT1 were significantly upregulated in NPC patients compared with healthy control group (all P<0.001). The plasma MALAT1 level was associated with N classification. The AUCs of NAET1, H19 and MALAT1 were 0.715, 0.708 and 0.704, respectively. Combination of NEAT1 and H19 could provide a more effective diagnostic performance (AUC: 0.734).ConclusionThe plasma lncRNA NEAT1, H19 and MALAT1 may be potential biomarkers for NPC serological diagnosis.