HIF-1α、ERα和MMP-9在甲状腺乳头状癌中的表达及临床意义

Expressions and clinical significances of HIF-1α，ERα and MMP-9 in papillary thyroid carcinoma

摘要:

目的探讨乏氧因子-1α（hypoxia inducible factor1α, HIF-1α）、雌激素受体α（estrogen receptor α, ERα）与基质金属蛋白酶-9（matrix metalloproteinases9, MMP-9）在甲状腺乳头状癌中的表达及与临床因素的关系。方法采用免疫组化技术对88例乳头状癌及癌旁组织中HIF-1α、ERα和MMP-9的表达情况进行检测，分析三者与临床参数的关系。结果甲状腺乳头状癌（papillary thyroid carcinoma,PTC）组织中HIF-1α、ERα和MMP-9三者阳性率（70.45%，62.5%和84.1%）均高于癌旁组织（P<0.01）；PTC组织中HIF-1α、ERα和MMP-9阳性率与淋巴结转移和临床分期有关。在淋巴结转移组和临床Ⅲ~Ⅳ期病例3者阳性率均高于无淋巴结转移组和临床I~Ⅱ期病例（P<0.05或P<0.01）；ERα阳性率在直径>2 cm的病例高于直径≤2 cm者（P<0.05）；HIF-1α与ERα、HIF-1α与MMP-9阳性率呈正相关（均为P<0.01）；ERα阳性率与MMP-9也呈正相关（P<0.01）。结论甲状腺乳头状癌中HIF-1α、ERα高表达可能通过MMP-9参与PTC的临床进程。

关键词:乏氧因子-1α|雌激素受体α|基质金属蛋白酶9|甲状腺乳头状癌|免疫组化

Abstract:

Abstract:ObjectiveTo exlpore the expressions and clinical significances of hypoxia inducible factorl alpha (HIF1α), estrogen receptor alpha (ERα) and matrix metalloproteinases9 (MMP9) in papillary thyroid carcinoma(PTC).MethodsThe expressions of abovementioned factors in specimens of PTC and paracarcinoma thyroid tissues from 88 PTC patients were detected by immunohistochemical method (IHC). And the relationships among clinical parameters and their expressions were analyzed.ResultsThe positive rates of HIF1α，ERα and MMP9 proteins in PTC were 70.45%, 62.5% and 84.1% respectively, which were higher than those of the paracarcinoma tissues (all P<0.01). And their positive rates in the specimens with lymph node metastasis or at clinical stage Ⅲ～Ⅳ were higher than those without lymph node metastasis or at clinical stage Ⅰ～Ⅱ (P<0.05 or P<0.01), which indicated that their positive rates were related to the lymph node metastasis and clinical stage of PTC. The positive rate of ERαin specimens with diameter more than 2 cm was higher than that in those with diameter equal to or less than 2 cm (P<0.05). Their positive rates were positively correlated with each other (all P<0.01).ConclusionEnhanced expressions of HIF1α and ERαmay participate in the clinical course of PTC via the high expression of MMP9.

Key words:Hypoxia inducible factorl alpha|Estrogen receptor alpha|Matrix metalloproteinases9|Papillary thyroid carcinoma|Immunohistochemistry