白细胞介素25与白细胞介素13及血管 内皮生长因子在鼻息肉组织中的表达

Expressions of Interleukin25, Interleukin13 and vascular endothelial growth factor in nasal polyp tissues

摘要:

目的通过观察慢性鼻-鼻窦炎伴有鼻息肉（CRSw NP）患者鼻息肉组织中白细胞介素25（interleukin25，IL25）、白细胞介素13（interleukin13，IL13）和血管内皮生长因子（vascular endothelial growth factor，VEGF）的表达及分布，来探讨其在鼻息肉发病中的作用及三者的相关性。方法选取30例在鼻内镜下行手术治疗的慢性鼻-鼻窦炎伴有鼻息肉患者鼻息肉组织作为实验组，同期选取30例因鼻中隔偏曲而行下鼻甲部分切除患者的下鼻甲黏膜组织作为对照组。应用免疫组织化学SP法来检测两组鼻黏膜组织中IL25、IL13及VEGF的表达情况，并采用直线相关分析和χ2检验进行统计学分析。结果免疫组化结果显示，IL25、IL13及VEGF在鼻息肉组织中的阳性细胞计数分别为（21.0±12.08、23.94±18.23、19.13±9.85），均高于对照组（4.19±2.54、7.67±2.02、3.44±1.52），两两比较差异均具有统计学意义（P<0.05）；3个因子在鼻息肉组织中的表达两两均呈正相关性（P<0.01）。结论IL25、IL13及VEGF在鼻息肉组织中的表达均升高，在鼻息肉的发病中可能起重要作用；鼻息肉组织中IL25、IL13及VEGF的表达两两均呈正相关，提示细胞因子之间的相互作用对鼻息肉发生起促进作用；以细胞因子及其受体为靶点，可能对开拓治疗鼻息肉的新方法提供理论依据。

关键词:白细胞介素-25|白细胞介素-13|血管内皮生长因子|鼻息肉|免疫组织化学

Abstract:

ObjectiveTo explore the roles and correlations of interleukin25 (IL25), interleukin13 (IL13) and vascular endothelial growth factor (VEGF) in nasal polyps by observing their expressions and distributions in nasal polyps of patients suffering from chronic rhinitis sinusitis with nasal polyps (CRSw NP).Methods30 cases suffering from CRSw NP and received endoscopic surgical treatment were chosen as experimental group while 30 cases received partial inferior turbinectomy due to nasal septum deviation as control. Specimens of nasal polyp and mucosa of inferior turbinate were obtained from the experimental group and the control group respectively. Immunohistochemical SP method was adopted to detect their expressions in all the specimens. Linear correlation analysis and χ2 test were used for statistical analysis.ResultsImmunohistochemistry showed that the positive cells of IL25, IL13 and VEGF in the experiment group were 21.0±12.08, 3.94±18.23 and 19.13±9.85 respectively, which were higher than those of the control group (4.19± 2.54, 7.67±2.02 and 3.44±1.52). Pairwise comparisons revealed statistically significant differences (all P<0.05). The expressions of the three factors in nasal polyps were positively correlated with each other (all P<0.01).ConclusionsThe expressions of IL25, IL13 and VEGF in nasal polyps are enhanced, which may play important roles in the pathogenesis of nasal polyps. The positive correlations between the expression of the three factors with each other in nasal polyps suggest that cytokines interaction may promote the occurrence of nasal polyps. Targeting cytokines and their receptors as potential therapeutic strategy may provide theoretical bases for developing new ways for the treatment of nasal polyps.

Key words:Interleukin-25| Interleukin-13|Vascular endothelial growth factor| Nasal polyps| Immunochemistry