鼻咽癌组织中Shh与微血管密度的表达及其 和肿瘤转移的关系

The relationship of Shh expression and microvessel density with tumor metastasis in nasopharyngeal carcinoma

摘要:

目的分析鼻咽癌组织中Shh蛋白的表达以及与微血管密度分布的关系，探讨其与肿瘤淋巴结转移的关系。方法应用免疫组化Elivision PIus二步法，检测50例鼻咽癌和26例炎性鼻咽部黏膜组织间质微血管密度(micro vascular density，MVD)的分布，并检测Shh的表达。结果①Shh在鼻咽癌组织中较鼻咽慢性炎症组织明显呈高表达(P<0.05)；②鼻咽癌中Shh阳性组MVD均值26.441±6.239,高于Shh阴性组15.667±4.899,两者之间差异有显著性(P<0.01);肿瘤细胞Shh表达与MVD相关,即MVD值随着Shh表达而增高；③鼻咽癌组织中Shh蛋白表达率与MVD均与临床分期，颈淋巴结转移有密切关系(P<0.05)。结论Shh可能促进鼻咽癌间质血管生成，促进肿瘤的侵袭和转移，Shh和MVD有可能成为判定鼻咽癌生物学行为和预后的重要指标。

关键词:鼻咽肿瘤|Shh蛋白|微血管密度|免疫组织化学

Abstract:

ObjectiveTo investigate the relationship between the expression of Shh protein and microvessel density in nasopharyngeal carcinoma (NPC), and to explore their relationships with tumor metastasis.MethodsImmunohistochemical twostep Elivision Plus method was adopted to detect the microvessel density (MVD) distribution and Shh expression in specimens from 5 cases of NPC and 26 patients with inflammation of nasal pharyngeal mucosa.Results①The expression of Shh in NPC was higher than that in chronic nasopharyngitis (P<0.05); ②The mean MVD value in NPC group (26.441±6.239) was higher than that of the nasopharyngitis (15.667±4.899), and the difference was statistically significant (P<0.01). The expression of Shh was positively related with MVD. ③The expression rate of Shh and MVD distribution in NPC tissue were closely related to the clinical stage and lymph node metastasis of this tumor(P<0.05).ConclusionsShh may promote the interstitial angiogenesis and facilitate tumor invasion and metastasis in NPC. Shh and MVD may be important indexes to judge the biological behavior and prognosis of NPC.

Key words:Nasopharyngeal neoplasm|Shh protein|Microvessel density| Immunohistochemistry