鼻咽癌组织中Shh与微血管密度的表达及其 和肿瘤转移的关系

The relationship of Shh expression and microvessel density with tumor metastasis in nasopharyngeal carcinoma

摘要:

目的分析鼻咽癌组织中Shh蛋白的表达以及与微血管密度分布的关系，探讨其与肿瘤淋巴结转移的关系。方法应用免疫组化Elivision PIus二步法，检测50例鼻咽癌和26例炎性鼻咽部黏膜组织间质微血管密度(micro vascular density，MVD)的分布，并检测Shh的表达。结果①Shh在鼻咽癌组织中较鼻咽慢性炎症组织明显呈高表达(P<0.05)；②鼻咽癌中Shh阳性组MVD均值26.441±6.239,高于Shh阴性组15.667±4.899,两者之间差异有显著性(P<0.01);肿瘤细胞Shh表达与MVD相关,即MVD值随着Shh表达而增高；③鼻咽癌组织中Shh蛋白表达率与MVD均与临床分期，颈淋巴结转移有密切关系(P<0.05)。结论Shh可能促进鼻咽癌间质血管生成，促进肿瘤的侵袭和转移，Shh和MVD有可能成为判定鼻咽癌生物学行为和预后的重要指标。

Abstract:

ObjectiveTo investigate the relationship between the expression of Shh protein and microvessel density in nasopharyngeal carcinoma (NPC), and to explore their relationships with tumor metastasis.MethodsImmunohistochemical twostep Elivision Plus method was adopted to detect the microvessel density (MVD) distribution and Shh expression in specimens from 5 cases of NPC and 26 patients with inflammation of nasal pharyngeal mucosa.Results①The expression of Shh in NPC was higher than that in chronic nasopharyngitis (P<0.05); ②The mean MVD value in NPC group (26.441±6.239) was higher than that of the nasopharyngitis (15.667±4.899), and the difference was statistically significant (P<0.01). The expression of Shh was positively related with MVD. ③The expression rate of Shh and MVD distribution in NPC tissue were closely related to the clinical stage and lymph node metastasis of this tumor(P<0.05).ConclusionsShh may promote the interstitial angiogenesis and facilitate tumor invasion and metastasis in NPC. Shh and MVD may be important indexes to judge the biological behavior and prognosis of NPC.