MiR-98靶向MTDH调控鼻咽癌恶性进展的实验研究

MiR-98 regulates the malignant progression of nasopharyngeal carcinoma via targeting MTDH

摘要:

目的探讨miR98对鼻咽癌细胞体外增殖和侵袭能力的影响及其可能机制。方法利用脂质体介导的miR98 mimic（及阴性对照）转染鼻咽癌CNE1和CNE2细胞，CCK8检测其体外增殖能力的改变，划痕愈合及Transwell侵袭小室实验检测鼻咽癌细胞的体外迁移及侵袭潜能变化，生物信息学软件预测miR98可能的靶基因，并经双荧光素酶及Western blotting验证靶基因。结果MiR98过表达能抑制鼻咽癌CNE1和CNE2细胞的体外生长增殖、迁移及侵袭能力；miR98对MTDH具有直接靶向调控作用。结论本研究表明miR98能够靶向MTDH调控鼻咽癌细胞株的体外生长增殖及侵袭能力。

关键词:鼻咽癌|MiR-98|MTDH;增殖|侵袭

Abstract:

ObjectiveTo investigate the effect and mechanism of miR98 on the proliferation and invasion in nasopharyngeal carcinoma (NPC) in vitro.MethodsLiposomemediated MiR98 mimic and negative control were applied to transfect CNE1 and CNE2 cells. CCK8 assay was used to examine the proliferation of the cells in vitro. Alterations of migration and invasion were assayed by scratches and transwell chambers. Target genes of miR98 were predicted by online target gene prediction database. Dual luciferase reporter assay and western blotting were used to validate their binding.ResultsMiR98 mimic successfully decreased capacities of growth, proliferation, migration and invasion in both CNE1 and CNE2 cell lines. Meanwhile, MTDH was a direct target gene of miR98.ConclusionMiR98 inhibits the growth, proliferation, migration and invasion of NPC cell lines by targeting MTDH.

Key words:Nasopharyngeal neoplasma| MiR-98| MTDH| Proliferation|Invasion