鼻咽癌组织中乏氧诱导因子1α和血管内皮生长因子的表达及其临床意义

Expressions of hypoxia inducible factor-1α and vascular endothelial growth factor in nasopharyngeal carcinoma and their clinical significances

摘要:

目的研究乏氧诱导因子1α (hypoxiainducible factor1α，HIF1α) 及血管内皮生长因子(vascular endothelial growth factor，VEGF)在鼻咽癌组织中的表达情况，并分析其相关性及与患者预后的关系。方法运用免疫组化染色及评分方法检测56例鼻咽癌患者及18例鼻咽部慢性炎症患者鼻腔黏膜组织中HIF1α、VEGF的表达差异，并进一步分析HIF1α、VEGF表达水平与鼻咽癌患者临床病理指标之间的关系。 结果免疫组化结果显示，在56例鼻咽癌患者中，HIF1α蛋白表达阳性率为53.6%（30/56），其中“-”26例，“+”11例，“++”12例，“+++”7例；VEGF蛋白表达阳性率为67.9%（38/56），其中“-”18例，“+”17例，“++”13例，“+++”8例；在18例鼻咽部慢性炎症患者中，HIF1α和VEGF表达阳性率分别为22.2%（4/18）和27.8%（5/18）。同时，在鼻咽癌患者中，HIF1α、VEGF的表达均与患者年龄、性别、分化程度无相关性（P>0.05），而与临床分期、T分级、淋巴结转移具有相关性（P<0.05）。Spearman分析显示鼻咽癌组织中HIF1α表达与VEGF表达呈正相关。结论鼻咽癌组织中存在HIF1α及VEGF的高表达，HIF1α及VEGF在鼻咽癌组织中的表达呈正相关，协同促进肿瘤新血管形成。

关键词:乏氧诱导因子1α; 血管内皮生长因子|鼻咽癌|免疫组化|临床意义

Abstract:

Abstract:ObjectiveTo detect the expressions of hypoxiainducible factor1α (HIF1α) and vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma (NPC) tissues, and to investigate their relationships and clinical significances.MethodsImmunohistochemistry was used to detect the expression levels of HIF1α and VEGF in 56 specimens of NPC and 18 of normal nasal mucosal tissue. The relationships between expression of HIF1α and that of VEGF, their expressions and clinicopathological features of NPC were analyzed.ResultsIn NPC, the positive rate of HIF1α was 53.6%(30/56), including “+” in 11 cases，“++” in 12 and “+++” in 7. The positive rate of VEGF was 67.9%(38/56) including “+” in 17 cases, “++” in 13, and “+++” in 8. The positive rates of HIF1α and VEGF in normal nasal mucosa were 22.2%(4/18) and 27.8%(5/18), respectively. In NPC, the expressions of HIF1α and VEGF expression had no correlation with patients’ age, gender and differentiation degree of tumor(all P>0.05), but were correlated with clinical stage, T stage and lymphatic metastasis of tumor(all P<0.05). Spearman analysis showed that HIF1α expression was positively correlated with that of VEGF in NPC.ConclusionHIF1α and VEGF are overexpressed in NPC. Their positively correlated overexpressions may have a synergistic effect on the neovascularization of NPC.

Key words:Hypoxiainducible factor1α|vascular endothelial growth factor|Nasopharyngeal neoplasm| Immunohistochemistry|Clinical significance