WT1基因在头颈部鳞状细胞癌中的表达及临床意义

Expression and clinical significance of WT1 gene in head and neck squamous cell carcinoma

摘要:

目的检测头颈部鳞状细胞癌（head and neck squamous cell carcinoma, HNSCC）组织中的Wilms’tumor gene 1（WT1）基因在mRNA及蛋白水平的表达情况，分析其与各临床病理特征的关系。方法应用实时荧光定量PCR法检测46例HNSCC组织及20例正常咽部黏膜组织中的WT1 mRNA表达情况，同时应用免疫组织化学法检测两组石蜡切片中的WT1蛋白表达情况。结果① 46例HNSCC组织WT1mRNA表达均值为9.61×10-3，较正常咽部黏膜组均值1.18×10-4 显著增高(P<0.05)；② WT1mRNA表达与肿瘤的分化程度显著相关，分化越差表达越高(P<0.05)，且WT1表达在下咽癌组织及嗜酒人群中表达亦显著增高(P<0.05)；③ WT1蛋白在HNSCC组织中阳性率为76.09%，而正常咽部黏膜组织无表达，两者差异具有统计学意义(P<0.05)。结论HNSCC组织中WT1在mRNA及蛋白水平均有异常过表达，可能扮演癌基因的角色，参与了肿瘤的发生和发展。

关键词:WT1基因|头颈部鳞状细胞癌|实时荧光定量PCR|免疫组织化学

Abstract:

Abstract:ObjectiveTo investigate the expression of Wlims’ tumor gene 1(WT1) in head and neck squamous cell carcinoma (HNSCC) at mRNA and protein level, and to analyze their relationships with clinicopathological characteristics of this tumor.MethodsThe expression of WT1 mRNA was detected by realtime fluorescence quantitative PCR method in 46 specimens of HNSCC tissues and 20 of normal pharyngeal mucosa tissues. The expression of WT1 protein in paraffin sections of two groups was detected by immunohistochemical technology.Results①The mean expression level of WT1 mRNA was 9.61×10-3 in HNSCC, which was significantly higher than that of normal pharyngeal mucosa tissues(1.19×10-4) (P<0.05). ②High WT1 expression level in HNSCC significantly correlated with poor differentiation of tumor (P<0.05). Furthermore, high expression level of WT1 mRNA was observed in specimens of hypolaryngeal carcinoma (P<0.05) and heavy drinkers (P<0.05) compared with tumors of other locations. ③WT1 was positively expressed at protein level in 35 of 46 HNSCC specimens (76.09%), which was significantly higher that of specimens of normal pharyngeal mucosa (0/20,0%).The difference was statistically significant (P<0.05).ConclusionWT1 gene is overexpressed at both mRNA and protein level in HNSCC, which suggests its important role in pathogenesis of HNSCC.

Key words:Wilms’ tumor gene 1(WT1)|Head and neck squamous cell carcinoma(HNSCC)|Realtime quantitative reverse transcription polymerase chain reaction(RTPCR)|Immunohistochemistry