MiR-324-3p经上皮-间质转化调控鼻咽癌迁移侵袭的实验分析

MiR-324-3p regulates the migration and invasion of nasopharyngeal carcinoma via epithelialmesenchymal transition

摘要:

摘要：目的探讨miR3243p对鼻咽癌细胞体外迁移侵袭能力的影响。方法MiR3243p转移上调其在鼻咽癌58F和610B细胞中的表达，采用划痕愈合实验及Transwell侵袭实验检测上调后的鼻咽癌58F和610B细胞迁移及侵袭能力；western blot检测上皮-间质转化（EMT）上皮细胞标志物Ecadherin和间质细胞标志物Vimentin的表达。结果MiR3243p成功转移上调了其在鼻咽癌58F和610B细胞中的表达。过表达miR3243p的鼻咽癌58F和610B细胞的体外迁移及侵袭能力显著下降，可同时伴随着Ecadherin的表达上调，Vimentin的表达下降。结论MiR3243p可能通过诱导EMT改变调控鼻咽癌细胞的侵袭转移能力。

关键词:鼻咽癌|miR-324-3p|侵袭|转移|上皮-间质转化

Abstract:

Abstract:ObjectiveTo investigate the effect of miR3243p on the migration and invasion in nasopharyngeal carcinoma (NPC) in vitro.MethodsMiR3243p mimic was used to upregulate the expression of miR3243p in NPC 58F and 610B cell lines, and then alterations of migration and invasion were examined by wound healing assays and Transwell invasion assays. Molecular markers correlated with epithelialmesenchymal transition (EMT) were finally tested.ResultsThe expression of miR3243p was successfully upregulated in both NPC 58F and 610B cells. Moreover, miR3243p overexpression led to enhanced capabilities of migration and invasion in NPC 58F and 610B cells, which was accompanied by elevated expression of Ecadherin and decreased expression of Vimentin.ConclusionMiR3243p can regulate the migration and invasion of NPC via EMT.

Key words:Nasopharyngeal neoplasma; MiR3243p; Invasion|Metastasis| Epithelial-mesenchymal transition