增龄相关性听力损失大鼠耳蜗TMPRSS3和ENaC-α蛋白的表达

Expression of TMPRSS3 and ENaC-α in the cochlea of rats with age-related hearing loss

摘要:

目的：通过检测增龄相关性听力损失SD大鼠耳蜗中跨膜丝氨酸蛋白酶3（transmembrane protease, serine 3, TMPRSS3）、表皮钠通道α（epithelial sodium channel-a, ENaC-α）蛋白的表达，初步探讨其在增龄相关性听力损失中的作用。方法：选用3、12及24月龄SD大鼠各30只，应用免疫组化、Western blot技术检测各组大鼠耳蜗中TMPRSS3、ENaC-α蛋白表达水平。结果：随月龄增大，大鼠ABR阈值逐渐升高，耳蜗TMPRSS3、ENaC-α蛋白表达水平逐渐降低，差异具有统计学意义（P<0.01）。结论：增龄相关性听力损失大鼠的耳蜗TMPRSS3、ENaC-α蛋白随月龄增大而降低。ENaC-α蛋白的变化与TMPRSS3呈相关性，提示TMPRSS3可能通过调节ENaC-α蛋白而起作用。

Abstract:

Objective：To explore the role of TMPRSS3 and ENaC-α in sensorineural deafness via investigating the expression in the cochlea of SD rats with age-related hearing loss. MethodsRats used for age-related hearing loss were divided into three groups, for 3-month-old group, 12-month-old group and 24-month-old group. The auditory function was evaluated by evoked auditory brainstem responses (ABR). The expression of TMPRSS3 and ENaC-α in the cochlea was detected by Paraffin-embedded immunohistochemistry and Western blot assay. ResultsABR threshold increased and expression of TMPRSS3, ENaC-α decreased with aging in the cochlea of SD rats. ConclusionsThe expression of TMPRSS3, ENaC-α decreases with aging in the cochlea of SD rats. The change of ENaC-α expression has some correlation with TMPRSS3 which indicates that TMPRSS3 may regulate the expression of ENaC-α.