鼻咽癌患者唾液和血清及外周血白细胞中EBV-DNA的定量检测

Quantitative analysis of EpsteinBarr virus DNA in saliva, blood serum and peripheral blood cells in patientswith nasopharyngeal carcinoma

摘要:

目的 对鼻咽癌患者唾液、血清及外周血白细胞中的EBV-DNA进行检测，分析唾液、血清、外周血白细胞中EB病毒的DNA含量与鼻咽癌的相关性。方法 对40例初诊鼻咽癌患者和50例正常人的唾液、血清及外周血白细胞中的EBV-DNA进行荧光聚合酶链反应（FQ-PCR）检测。结果 EBV-DNA在鼻咽癌患者血清中的检出率为67.5%，显著高于正常人血清中的EBV-DNA检出率2%（χ2=44.48，P<0.001）；鼻咽癌患者的唾液、外周血白细胞EBV-DNA检出率与正常人相比，无显著性差异；鼻咽癌患者和正常人的唾液EBV-DNA拷贝数与外周血白细胞EBVDNA拷贝数呈正相关性；鼻咽癌患者血清EBV-DNA拷贝数与TNM分级有关，分级越晚含量越高。结论 EB病毒长期存在于多数人的唾液中，也可存在于外周血B淋巴细胞中。血清EBV-DNA检测在鼻咽癌的临床诊断中具有较高的敏感性和特异性，有助于鼻咽癌的早期诊断；血清EBVDNA拷贝数与TNM分级有关，可在分子水平对鼻咽癌的TNM分级进行补充。 

Abstract:

Objective〓〖HQK〗〖WTBZ〗To quantitatively analyze DNA levels of EpsteinBarr virus (EBV) in saliva, blood serum, and peripheral blood cells (PBCs) of nasopharyngeal carcinoma (NPC) patients, and to investigate the relationship between EBV-DNA levels and NPC. Methods EBV-DNA levels in samples of saliva, blood serum and PBCs from 40 primary NPC patients and 50 controls were quantitatively detected with realtime quantitative PCR (FQ-PCR) respectively. Results The detective rate of EBV-DNA in blood serum from NPCs (67.5%) was significantly higher than that in the controls 2% (χ2=44.48, P<0.001). The differences of EBV-DNA detective rates in the saliva, PBCs between the NPCs and control group were statistically insignificant. Saliva EBV-DNA level was correlated with PBCs EBV-DNA load in both NPCs and the control. In NPC patients, blood serum EBV-DNA level was significantly increasing with the advance in TNM stage. Conclusion EpsteinBarr virus may exist in saliva and peripheral blood B lymphocyte in most normal people. With high sensitivity and specificity, the blood serum EBVDNA examination may be helpful in the early diagnosis of NPC, and also facilitate the TNM staging of NPC in clinical practice.〖HQ〗〖WT5”HZ〗