喉癌组织中微小RNA-129-2基因甲基化程度的研究

Methylation of MicroRNA-129-2 in Laryngeal Cancer

摘要:

目的 研究喉癌肿瘤组织中微小RNA1292基因（MiR-129-2）的甲基化程度及其与患者临床分级和癌组织病理分化程度的关系。方法 应用Methyl-ProfilerTM DNA甲基化PCR系统对12例喉癌肿瘤组织和6例癌旁组织进行甲基化分析。结果 喉癌组织中MiR-129-2高甲基化率为66.7%（8/12），癌旁组织中MiR-129-2高甲基化率为0（0/6），两组比较差异有统计学意义（P<0.05）。喉癌患者临床分级与MiR-129-2高甲基化率差异无统计学意义（P>0.05）。病理分化程度与MiR-129-2高甲基化率差异无统计学意义（P>0.05）。结论 MiR-129-2基因在喉癌组织中甲基化水平升高，MiR-129-2高甲基化水平可能与喉癌发生的病理机制有关。

关键词:

喉肿瘤|癌|微小RNA|甲基化

Abstract:

Objective To assess the methylation level of MicroRNA-129-2 (MiR-129-2) in laryngeal cancer. Methods We applied Methyl-ProfilerTM DNA PCR Array System (SABiosciences Company) to analysis of 12 laryngeal cancer and 6 paracancer tissues. Results Hypermethylation was detected in 66.7% of the cancer tissue (8/12) and none in paracancer tissue (0/6) (P<0.05); the difference was significant. Among the clinical cancer tissue of each clinical stage , there was no significant difference of MiR-129-2 hypermethylation rate (P>0.05). There was no significant difference of MiR129-2 hypermethylation rate between two pathologic grades (P>0.05). Conclusion The methylation level of MiR1292 is higher in laryngeal cancer tissue than in paracancer tissue, indicating that it may play a role in laryngeal cancer. The methylation level of MiR-129-2 may not be related with the clinical stage of laryngeal cancer. The methylation level of MiR-129-2 may not be related with the pathologic grade of laryngeal cancer.

Key words:

Laryngeal neoplasms|cancer|MicroRNA|Methylation