Survivin在鼻息肉组织中的表达及与嗜酸性粒细胞浸润相关性的研究

Survivin expression and eosinophil infiltration in nasal polyp and their correlation

摘要:

摘要：目的：研究生存素（Survivin）在鼻息肉组织中的表达及其与嗜酸性粒细胞浸润的相关性，探讨Survivin在鼻息肉发病过程中的作用。方法：收集28例鼻息肉组织（鼻息肉组）和12例正常下鼻甲组织（对照组），用HE染色法观察组织中的嗜酸性粒细胞浸润情况，免疫组化法检测组织中Survivin的表达。结果：① HE染色显示鼻息肉组嗜酸性粒细胞浸润显著增加。②免疫组织化学染色显示Survivin免疫阳性细胞数及着色强度均明显高于对照组，图像分析显示，鼻息肉组Survivin的积分光密度（IOD，103/HP）为50.21±6.32，与对照组5.67±0.58相比有统计学意义（P<0.05）。③ Spearman等级相关分析显示，鼻息肉中Survivin表达与嗜酸性粒细胞浸润表达密切相关（r=0.673，P<0.01）。结论：鼻息肉组织中Survivin表达、嗜酸性粒细胞浸润均上调，且二者有协同表达关系。鼻息肉可能是通过凋亡抑制基因Survivin使炎性细胞凋亡受到抑制，从而促进炎性细胞主要是嗜酸性粒细胞浸润生长，导致慢性炎症反应。

Abstract:

ABSTRACT:OBJECTIVE：To study Survivin expression and eosinophil infiltration in nasal polyp and their correlation, and to explore the role of Survivin in the pathogenesis of nasal polyp. METHODS:28 cases of nasal polyp and 12 cases of normal inferior turbinate mucosa were enrolled in this study. Immunohistochemistry method for Survivin and HE staining for eosinophils were performed in these two groups. RESULTS:① The number of eosinophil in the nasal polyp was higher than that in the control group by HE staining. ② The number and intensity of positive stained cells of Survivin in nasal polyp were higher than those in the control group. Image analysis showed that IOD of Survivin（103/HP）in the nasal polyp was 50.21±6.32 while that of the control group was 5.67±0.58. The differences between the two groups were statistically significant (P<0.05). ③ Spearman correlation test showed that there was a close correlation between Survivin expression and eosinophil infiltration in nasal polyp (r=0.673, P<0.01). CONCLUSION:Both Survivin and eosinophil are over expressed in nasal polyp with close correlation.Survivin can inhibit apoptosis of inflammatory cells including eosinophil to promote infiltration and growth of inflammatory cells, therefore lead to the chronic inflammatory reaction in nasal polyp.